https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44957 Tue 25 Oct 2022 12:35:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29462 IRAKM, PCDH1, ORMDL3/GSDMB, IL-33, CDHR3 and CST1 in airway epithelial cells. Recent studies on epigenetic regulatory factors have further provided novel insights to the field, particularly their effect on regulation of some of the asthma susceptibility genes (e.g. methylation of ADAM33). Among the epigenetic regulatory mechanisms, microRNA networks have been shown to regulate a major portion of post-transcriptional gene regulation. Particularly, miR-19a may have some therapeutic potential.]]> Thu 24 Mar 2022 11:32:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46730 Thu 01 Dec 2022 10:28:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27531 Sat 24 Mar 2018 07:28:58 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22016 Sat 24 Mar 2018 07:15:45 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21998 Sat 24 Mar 2018 07:14:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23404 IIbß₃, irreversible platelet aggregation and thrombus stabilisation. Tetraspanin superfamily member CD151 associates with integrin a_IIbß₃ and plays critical roles in regulation of thrombus growth and stability in vivo. The possible functional relationship between P2Y₁₂ and CD151 in a molecular cluster in platelets may affect thrombus formation. Hence our aim was to investigate the physical and functional requirements for this association in platelets. Our investigations reveal a specific and constitutive association between CD151 and P2Y₁₂ receptor in human platelets shown by immunoprecipitation/western blot studies and by flow cytometry. Specifically, the prominent association involves CD151 with P2Y₁₂ oligomers, and to a lesser extent P2Y₁₂ monomers. This association is not altered by platelet aggregation induced by different agonists. There is also a distinct complex of tetraspanin CD151 with ADP purinergic receptor P2Y₁₂ but not P2Y₁. P2Y₁₂ oligomer interaction with CD151 is selective as compared to other tetraspanins. To investigate the functional relationship between these receptors in platelets we used wild-type or CD151 knockout (KO) mice treated with either PBS or 50 mg/kg clopidogrel. CD151 KO mice treated with clopidogrel exhibited synergy in delayed kinetics of clot retraction, in PAR-4 and collagen-mediated platelet aggregation, platelet spreading on fibrinogen and without restricting cAMP inhibition. Clopidogrel treated CD151 KO arterioles showed smaller and less stable thrombi with increased tendency to embolise ex vivo and in vivo. These studies demonstrate a complementary role between CD151 and P2Y₁₂ receptor in platelets in regulating thrombus growth and stability.]]> Sat 24 Mar 2018 07:13:56 AEDT ]]>